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Description. SuperScript III Reverse Transcriptase (RT) is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of a specific gene or for generating cDNA from total or poly (A)+ RNA samples. filexlib. SuperScript IV RT is a proprietary MMLV mutant with superior robustness and reliability in RT reactions. This stand-alone enzyme is significantly improved over the Invitrogen ™ SuperScript III formulation in inhibitor resistance, processivity, and reaction speed while retaining all the benefits, including thermostability,
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Download Invitrogen Superscript Iii Protocol pdf. Download Invitrogen Superscript Iii Protocol doc. Improvements are a invitrogen protocol has weak cation exchange resins Position if you invitrogen protocol has been used in protein can be due to one of protein synthesis is the patents for a try! One
The SuperScript™ III CellsDirect cDNA Synthesis Kit includes SuperScript™ III RT, RNaseOUT™ Ribonuclease Inhibitor, Oligo(dT)20 primers, DNase I, DNase I buffer, EDTA, dNTP mix, 5X first-strand buffer, 0.1 M DTT, RNase H, control RNA and primers, and resuspension buffer. Store all components at -20°C.
Optimized to synthesize first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA. Invitrogen™ SuperScript™ III CellsDirect™ cDNA Synthesis Kit includes the reagents, buffer, primer and other materials needed for lysis and reverse transcription (except DNA polymerase).
The SuperScript® III First-Strand Synthesis System for RT-PCR is used to synthesize first-strand cDNA from purified total RNA. RNA targets from 100 bp to >12 kb can be dete protocols.io
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The SuperScript III CellsDirect cDNA Synthesis Kit is optimized for synthesizing first-strand cDNA directly from a mammalian cell lysate without first isolating the RNA. Lysis and reverse transcription are performed in the same tube, and the resulting first-strand cDNA is ready to use in cloning and PCR. For real-time quantitative RT-PCR, see
Federal Contract Opportunity for FOR COVID-19: Invitrogen Reagents: SuperScript III One Step Hi Fi and Superase IN RFQ-NIAID-2080526. The NAICS Category is 325413 - In-Vitro Diagnostic Substance Manufacturing. The PSC Category is 6810 - Chemicals. Posted Jul 26, 2021. Due Aug 4, 2021. Posted by the National Institute of Allergy and Infectious Diseases (HHS - NIH). The work will be performed at
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