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Millingtonia hortensis reversed senescence on H 2 O 2 -induced aging in SK-N-SH cells. The result showed that H 2 O 2 promoted a high number of SA-β-gal-positive cells (blue) approximately 50% [ Figure 6a ]. Treatment with MH significantly decreased of SA-β-gal-positive cells ( P < 0.001). The fresh stem barks of Millingtonia hortensisLinn were collected from Botanical garden of VIT University in Vellore district in Tamil Nadu, India during the month of January. The stem was washed thoroughly two to three times with running tap water and once with sterile distilled water and shade dried at room temperature. Millingtonia hortensis Linn. f has Bignonia suberosa Roxb. as synonym. The ornamental tree which has a telugu name also. It is quite probable that species collected by Koenig much earlier in his voyage to Siam has been described by Linnaeus filius just like Tectona from Burma, Pterocarpus santalinus from Tirupati hills by Koenig who is mentor of Roxburgh describing plants from Coromanedl. Millingtonia hortensis. Taxonavigation . Taxonavigation: Lamiales Classification System: APG IV Millingtonia in Kew Science Plants of the World online. The Board of Trustees of the Royal Botanic Gardens, Kew. Published online. Accessed: 2019 Apr. 27. Reference page. International Plant Names Index. 2019. Millingtonia. Published online. Accessed: Apr. 27 2019. The Plant List 2013 Fig 1: Habitat of Millingtonia hortensis 2. Materials and Methods 2.1 Collection of leaves and preparation of extract The leaves of M. hortensis were collected from Thiruvanmiyur, Chennai. The leaves were washed with tap water, shade dried for 10 d and course powdered by mechanical blender [6]. Twenty gram of leaves powder was Mahasarkham University Abstract Millingtonia hortensis Linn. flowers have been extracted for essential oil using vapor distillation, with 0.5-2% yield. The essential oil of M. hortensis Linn. was