Create your own fluorescence-based biomolecule quantification assay for the Qubit® 3.0 Fluorometer using the MyQubit assay design tool. I am using the Qubit 3.0 and we only had a RNA high sensitivity kit and I needed to test a sample with a concentration of 239ng/uL (read first on the Nanodrop 2000). I did a 1:5 dilution of this 9 Proceed to Reading Standards and Samples for the appropriate instrument. Reading Standards and Samples with the Qubit 3.0 Fluorometer 10 On the home screen of the Qubit, press dsDNA, then select dsDNA: High Sensitivity or dsDNA: Broad Range depending on the kit being used. 11 If calibration is being performed, press Read Standards to proceed. (If a calibration is not For use on Thermo Fisher Qubit™ 3.0 / 4 fluorometers, dilute the 25 ng/µL standard by adding 6 µL of the standard to 4 µL of the assay buffer to make the 15 ng/µL standard. For each standard or unknown sample, add 190 µL of the working solution into a labeled tube. Adjust volume when adding more or less than 10 µL of the unknown sample. As well as RNA-seq, it be applied to differential signal analysis of other types of genomic data that Broad GDAC Firehose A modification was done on the manual extractions by adding 280 µl per Measurement Data for Thermo Fisher Qubit™ 3.0 / 4 Fluorometers. Three replicates of RNA samples were measured on Thermo Fisher Qubit™ 3.0. and Qubit™ 4 fluorometers and a DeNovix DS-11 FX Fluorometer using the DeNovix RNA Assay. The data is presented in graphical and tabular form across the assay dynamic range below. Key features of the Qubit™ 3.0 Fluorometer include: Powerful dual-core processor quickly and accurately quantifies DNA, RNA, and protein in less than 5 seconds per sample Uses as little as 1 μL of sample Stores up to 1000 sample results Large, 5.7-inch, state-of-the-art color touch screen for easy workflow navigation Small footprint saves Qubit assays for use with the fluorometer are all performed using the same general protocol, which uses a simple mix-and-read format with incubation times of only two minutes; Advanced optics and data analysis algorithms; USB thumb drive and cable for data transfer to Excel™ sorftware and for software downloads; Applications: I am using the Qubit 3.0 and we only had a RNA high sensitivity kit and I needed to test a sample with a concentration of 239ng/uL (read first on the Nanodrop 2000). The Qubit 3.0 manual does DNA concentration was measured by a Qubit 3.0 fluorometer and expressed in ng/µL. Data points are averages of a duplicate with the SD values shown Techniques Used: DNA Extraction, Concentration Assay 2) Product Images from "Automated Workflow for Somatic and Germline Next Generation Sequencing Analysis in Routine Clinical Cancer Diagnostics"