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Download chapter PDF High-Performance Thin-Layer Chromatography (HPTLC) is a sophisticated and automated form of thin-layer chromatography. Modern instrumental HPTLC is a powerful analytical method equally suitable for qualitative and quantitative analytical tasks. This has been demonstrated by many publications and research projects. Abstract. The present study was aimed to identification, isolation, and quantification of marker in R. tuberosa (Acanthaceae). HPTLC fingerprinting was carried out for various extract of root, stem, and leaf of R. tuberosa.From the HPTLC fingerprint the florescent band (under 366 nm) at : 0.56 (mobile phase chloroform : toluene : ethyl acetate (6 : 3 : 1, v/v)) was found in leaf, root, and HPTLC finger print profile of n-hexane extract of Rauwolfia tetraphylla Linn Vinay K. N1, V. Venkata Lakshmi2*, constant application rate of 20 μl of sample was applied on 8mm wide band using High-Performance Thin-Layer Chromatography for the Analysis of Medicinal Plants presents the theoretical and technical information needed to perform reliable and reproducible high-performance validation, automation, and qualitative and quantitative analysis; applications of HPTLC separation with special reference to phytochemical analysis, biomedical analysis, herbal drug quantification, analytical analysis, finger print analysis; and HPTLC future to combinatorial approach, potential for hyphenation, HPTLC-MS, HPTLC-FTIR, and HPTLC-scanning diode laser. The chapters in the High-performance thin-layer chromatography ( HPTLC) is an enhanced form of thin-layer chromatography (TLC). A number of enhancements can be made to the basic method of thin-layer chromatography to automate the different steps, to increase the resolution achieved, and to allow more accurate quantitative measurements. TLC and HPTLC complement gas chromatography (GC) and high-performance column liquid chromatography (HPLC) for separation, detection, identification, and quantification of analyte because of their following unique advantages over column chromatography: (1) Single use of the layer simplifies sample preparation procedures. 5.4 Use 10 X 10 cm. HPTLC plate (Kieselgel 60F254 or Identical) 5.5 For Linearity of spotting 5.5.1 Apply 2 µl, 4µl, 6µl ,8µl and 10 µl of solution on HPTLC plate with spotter. 5.5.2 Allow the plate to run in mobile phase. 5.5.3 Dry the plate with drier. 5.5.4 Scan the plate with scanner. 5.5.5 Check the linearity and corelation coefficient. Product Release Information. Important information about this release. What's new in visionCATS 3.1. Overview of the new features. User documentation. The documentation for regular users of the visionCATS software. Installation and support. The information related to visionCATS installation, support and troubleshooting. visionCATS Guide. It allows the user to load the HPTLC Logger tree. Note In the built-in Full report template, the Load log data option is checked, but the Log option is unchecked by default in analysis. It means that, by default, the contents of the HPTLC Logger are available but not displayed. Outline ¶ The tree view displays the PDF