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Soil is variable environment with divers' microbial community consists of bacteria, actinomycetes , molds ,yeast, algae and protozoa. Necessary to use different In this work we focused to isolate the exo polysaccharide producing soil bacterium9. Materials and Methods. Soil sample collection. For bacterial isolation, 10 EXPERIMENT 4. ISOLATION OF SOIL BACTERIA: VIABLE TITER and PURE CULTURE. Bacteria in soil occur singly and in aggregates. To estimate the number of bacteriaTitle : Isolation of soil microorganisms. Objective : To isolate and count the microorganisms found in a sample of soil by the dilution method using.